TY - GEN
T1 - Automated biofilm region recognition and morphology quantification from confocal laser scanning microscopy imaging
AU - Zielinski, Jerzy S.
AU - Zielinska, Agnieszka K.
AU - Bouaynaya, Nidhal
AU - Vaughan, Justin G.
AU - Smeltzer, Mark S.
PY - 2011
Y1 - 2011
N2 - Staphylococcus aureus is an opportunistic human pathogen and a primary cause of nosocomial infections. Its biofilm forming capability is an adaptation strategy utilized by many species of bacteria to overcome stressful environmental conditions and provides both resistance to antimicrobial treatments and protection from the host immune system. This paper addresses a growing demand for an objective, fully automated method of biofilm structure description with standardized parameters that are independent of user input. In this study, we used watershed segmentation to analyze and compare confocal laser scanning microscopy (CLSM) images of two S. aureus strains with different biofilm-forming capabilities. Results are compared with manual calculations as well as the commonly used COMSTAT software.
AB - Staphylococcus aureus is an opportunistic human pathogen and a primary cause of nosocomial infections. Its biofilm forming capability is an adaptation strategy utilized by many species of bacteria to overcome stressful environmental conditions and provides both resistance to antimicrobial treatments and protection from the host immune system. This paper addresses a growing demand for an objective, fully automated method of biofilm structure description with standardized parameters that are independent of user input. In this study, we used watershed segmentation to analyze and compare confocal laser scanning microscopy (CLSM) images of two S. aureus strains with different biofilm-forming capabilities. Results are compared with manual calculations as well as the commonly used COMSTAT software.
UR - http://www.scopus.com/inward/record.url?scp=79959915436&partnerID=8YFLogxK
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U2 - 10.1109/BSEC.2011.5872327
DO - 10.1109/BSEC.2011.5872327
M3 - Conference contribution
AN - SCOPUS:79959915436
SN - 9781612844107
T3 - Proceedings of the 2011 Biomedical Sciences and Engineering Conference: Image Informatics and Analytics in Biomedicine, BSEC 2011
BT - Proceedings of the 2011 Biomedical Sciences and Engineering Conference
T2 - 2011 Biomedical Sciences and Engineering Conference: Image Informatics and Analytics in Biomedicine, BSEC 2011
Y2 - 15 March 2011 through 17 March 2011
ER -