Abstract
Staphylococcus aureus is an opportunistic human pathogen and a primary cause of nosocomial infections. Its biofilm forming capability is an adaptation strategy utilized by many species of bacteria to overcome stressful environmental conditions and provides both resistance to antimicrobial treatments and protection from the host immune system. This paper addresses a growing demand for an objective, fully automated method of biofilm structure description with standardized parameters that are independent of user input. In this study, we used watershed segmentation to analyze and compare confocal laser scanning microscopy (CLSM) images of two S. aureus strains with different biofilm-forming capabilities. Results are compared with manual calculations as well as the commonly used COMSTAT software.
Original language | English (US) |
---|---|
Title of host publication | Proceedings of the 2011 Biomedical Sciences and Engineering Conference |
Subtitle of host publication | Image Informatics and Analytics in Biomedicine, BSEC 2011 |
DOIs | |
State | Published - Jul 7 2011 |
Externally published | Yes |
Event | 2011 Biomedical Sciences and Engineering Conference: Image Informatics and Analytics in Biomedicine, BSEC 2011 - Knoxville, TN, United States Duration: Mar 15 2011 → Mar 17 2011 |
Other
Other | 2011 Biomedical Sciences and Engineering Conference: Image Informatics and Analytics in Biomedicine, BSEC 2011 |
---|---|
Country/Territory | United States |
City | Knoxville, TN |
Period | 3/15/11 → 3/17/11 |
All Science Journal Classification (ASJC) codes
- Computer Graphics and Computer-Aided Design
- Biomedical Engineering