Analysis of the intermediate size proteoglycans from the developing chick limb buds.

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Limb-bud proteoglycans are heterogeneous molecules which vary in their chemical and physical properties with development. This report describes proteoglycan intermediates (PG-I) that predominate in stage-34 limbs, and compares them with proteoglycan aggregates (PG-A) in stage-38 limbs. We analysed proteoglycans and their components extracted with guanidinium chloride by subjecting them to density gradient centrifugation, molecular sieve chromatography, electrophoretic separation, and selective enzymatic degradation. PG-I and PG-A have similar chondroitin sulphate composition, amino sugars, chondroitin sulphate side-chain length, glycoprotein link factors, and hyaluronic acid binding capacity, and both cross react with antisera prepared against cartilage-specific chick sternal proteoglycans. However, PG-I has lower molecular weight, lower buoyant density, and fewer chondroitin sulphate side chains on the protein core. The PG-I in the developing limb can be considered a mixture of smaller aggregates and cartilage-specific large monomers in which the former predominate.

Original languageEnglish (US)
Pages (from-to)61-74
Number of pages14
JournalJournal of Embryology and Experimental Morphology
StatePublished - Aug 1982
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology


Dive into the research topics of 'Analysis of the intermediate size proteoglycans from the developing chick limb buds.'. Together they form a unique fingerprint.

Cite this