Analysis of the efficiency of adenovirus transcription.

Cristina Iftode, S. J. Flint

Research output: Contribution to journalArticle

Abstract

This method is designed to measure rates of transcription from adenoviral promoters as a function of the concentrations within infected cells of the promoter(s) of interest. The latter parameter is assessed by quantification of viral DNA by hybridization of membrane-bound DNA following purification of DNA from nuclear fractions of adenovirus-infected cells. Two alternative protocols, primer extension and quantitative reverse transcription polymerase chain reaction, are described for determination of the concentrations of viral mRNAs purified from the cytoplasmic fractions of the same infected cell samples. An alternative procedure to measure rates of transcription directly using run-on transcription in isolated nuclei is also presented.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalMethods in molecular medicine
Volume131
DOIs
StatePublished - 2007

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

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