An activity transition from NADH dehydrogenase to NADH oxidase during protein denaturation

Scott Huston, John Collins, Fangfang Sun, Ting Zhang, Timothy D. Vaden, Y. H.Percival Zhang, Jinglin Fu

Research output: Contribution to journalArticlepeer-review

Abstract

A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN–DI) that happened during the long-term storage process. It was found that FMN–DI did not simply lose its β-nicotinamide adenine diphosphate (NADH) dehydrogenase activity after a long-time storage, but obtained a new enzyme activity of NADH oxidase. Further mechanistic studies suggested that the alteration of the binding strength of an FMN cofactor with a DI protein could be responsible for this functional switch of the enzyme.

Original languageEnglish (US)
Pages (from-to)286-293
Number of pages8
JournalBiotechnology and Applied Biochemistry
Volume65
Issue number3
DOIs
StatePublished - May 1 2018

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology

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