Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery

Nicholas M. Boulis; Aaron J. Noordmans; Debbie K. Song; Michael J. Imperiale; Adam Rubin; Paola Leone; Matthew During; Eva L. Feldman

doi:10.1016/j.nbd.2003.08.025

Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery

Nicholas M. Boulis, Aaron J. Noordmans, Debbie K. Song, Michael J. Imperiale, Adam Rubin, Paola Leone, Matthew During, Eva L. Feldman

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

The current investigation tests whether adeno-associated viral vectors (rAAV) undergo remote delivery to the spinal cord via peripheral nerve injection as previously demonstrated with adenoviral vectors. The sciatic nerves of adult rats (n = 10) were injected with either an rAAV (rAAVCMV-lacZ) or adenoviral (AdCMV-lacZ) vector (1.4 × 10⁷ particles/ml). After 21 days, the rAAV group demonstrated significantly higher spinal cord viral expression than the adenoviral group (P < 0.024). A second group of rats was injected with rAAV expressing the green fluorescence protein (GFP) reporter gene. GFP was detected 21 days after unilateral sciatic nerve injection in the neurons of the dorsal root ganglion and spinal cord. The codistribution of the viral genome and transgene in CNS neurons was confirmed with in situ hybridization. In summary, rAAV genes are expressed in CNS neurons following peripheral nerve injection at levels exceeding those seen following remote adenovirus injection.

Original language	English (US)
Pages (from-to)	535-541
Number of pages	7
Journal	Neurobiology of Disease
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/j.nbd.2003.08.025
State	Published - Dec 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neurology

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@article{626321b0867d40afbf62b1c969c9f37f,

title = "Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery",

abstract = "The current investigation tests whether adeno-associated viral vectors (rAAV) undergo remote delivery to the spinal cord via peripheral nerve injection as previously demonstrated with adenoviral vectors. The sciatic nerves of adult rats (n = 10) were injected with either an rAAV (rAAVCMV-lacZ) or adenoviral (AdCMV-lacZ) vector (1.4 × 107 particles/ml). After 21 days, the rAAV group demonstrated significantly higher spinal cord viral expression than the adenoviral group (P < 0.024). A second group of rats was injected with rAAV expressing the green fluorescence protein (GFP) reporter gene. GFP was detected 21 days after unilateral sciatic nerve injection in the neurons of the dorsal root ganglion and spinal cord. The codistribution of the viral genome and transgene in CNS neurons was confirmed with in situ hybridization. In summary, rAAV genes are expressed in CNS neurons following peripheral nerve injection at levels exceeding those seen following remote adenovirus injection.",

author = "Boulis, {Nicholas M.} and Noordmans, {Aaron J.} and Song, {Debbie K.} and Imperiale, {Michael J.} and Adam Rubin and Paola Leone and Matthew During and Feldman, {Eva L.}",

note = "Funding Information: We thank Bev Davidson, University of Iowa, and Jude Samulski, University of North Carolina, Chapel Hill, for supplying adenoviral and adeno-associated viral vectors, respectively. The present work was supported by NIH grants GM 34902, HL64762, T32 NS07222, and NS43305, The ALS Association, Hope for ALS, and the University of Michigan Center for Gene Therapy. ",

year = "2003",

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doi = "10.1016/j.nbd.2003.08.025",

language = "English (US)",

volume = "14",

pages = "535--541",

journal = "Neurobiology of Disease",

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TY - JOUR

T1 - Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery

AU - Boulis, Nicholas M.

AU - Noordmans, Aaron J.

AU - Song, Debbie K.

AU - Imperiale, Michael J.

AU - Rubin, Adam

AU - Leone, Paola

AU - During, Matthew

AU - Feldman, Eva L.

N1 - Funding Information: We thank Bev Davidson, University of Iowa, and Jude Samulski, University of North Carolina, Chapel Hill, for supplying adenoviral and adeno-associated viral vectors, respectively. The present work was supported by NIH grants GM 34902, HL64762, T32 NS07222, and NS43305, The ALS Association, Hope for ALS, and the University of Michigan Center for Gene Therapy.

PY - 2003/12

Y1 - 2003/12

N2 - The current investigation tests whether adeno-associated viral vectors (rAAV) undergo remote delivery to the spinal cord via peripheral nerve injection as previously demonstrated with adenoviral vectors. The sciatic nerves of adult rats (n = 10) were injected with either an rAAV (rAAVCMV-lacZ) or adenoviral (AdCMV-lacZ) vector (1.4 × 107 particles/ml). After 21 days, the rAAV group demonstrated significantly higher spinal cord viral expression than the adenoviral group (P < 0.024). A second group of rats was injected with rAAV expressing the green fluorescence protein (GFP) reporter gene. GFP was detected 21 days after unilateral sciatic nerve injection in the neurons of the dorsal root ganglion and spinal cord. The codistribution of the viral genome and transgene in CNS neurons was confirmed with in situ hybridization. In summary, rAAV genes are expressed in CNS neurons following peripheral nerve injection at levels exceeding those seen following remote adenovirus injection.

AB - The current investigation tests whether adeno-associated viral vectors (rAAV) undergo remote delivery to the spinal cord via peripheral nerve injection as previously demonstrated with adenoviral vectors. The sciatic nerves of adult rats (n = 10) were injected with either an rAAV (rAAVCMV-lacZ) or adenoviral (AdCMV-lacZ) vector (1.4 × 107 particles/ml). After 21 days, the rAAV group demonstrated significantly higher spinal cord viral expression than the adenoviral group (P < 0.024). A second group of rats was injected with rAAV expressing the green fluorescence protein (GFP) reporter gene. GFP was detected 21 days after unilateral sciatic nerve injection in the neurons of the dorsal root ganglion and spinal cord. The codistribution of the viral genome and transgene in CNS neurons was confirmed with in situ hybridization. In summary, rAAV genes are expressed in CNS neurons following peripheral nerve injection at levels exceeding those seen following remote adenovirus injection.

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Adeno-associated viral vector gene expression in the adult rat spinal cord following remote vector delivery

Abstract

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