Acute restraint increases NADPH-diaphorase staining in distinct subregions of the rat dorsal raphe nucleus: Implications for raphe serotonergic and nitrergic transmission

Chuma O. Okere, Barry D. Waterhouse

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The brainstem dorsal raphe nucleus (DRN) maintains a rough topographic cell ordering with respect to biological function. This study examined the influence of acute restraint on nitric oxide (NO) synthase (NOS) neurons in distinct DRN subregions. NADPH diaphorase staining (NOS index) intensity was higher in the DRN dorsomedial, ventromedial and lateral wings subregions of restrained vs. control rats. The mean number of cells was not significantly different between both groups of animals. The restrained-induced NADPH-diaphorase activity was significantly higher in the rostral ventromedial and caudal lateral wings than the corresponding caudal and rostral subregions but no significant difference was observed between rostral and caudal dorsomedial subregions. These observations suggest that restraint stress differentially activates NO-producing neurons in distinct DRN subregions.

Original languageEnglish (US)
Pages (from-to)174-181
Number of pages8
JournalBrain Research
Volume1119
Issue number1
DOIs
StatePublished - Nov 13 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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