Activation induced deaminase: The importance of being specific

Harold C. Smith; Andrea Bottaro; Mark P. Sowden; Joseph E. Wedekind

doi:10.1016/j.tig.2004.03.012

Activation induced deaminase: The importance of being specific

Harold C. Smith, Andrea Bottaro, Mark P. Sowden, Joseph E. Wedekind

Research output: Contribution to journal › Short survey › peer-review

8 Scopus citations

Abstract

Activation-induced deaminase (AID) is required for class switch recombination and somatic hypermutation in immunoglobulin genes. Although the preponderance of evidence suggests that AID functions by deaminating deoxycytidine in DNA, the question remains whether it can also deaminate cytidine in mRNA, as originally proposed based on its homology to RNA-editing enzymes. Recently, the biological relevance of assaying mammalian enzymes for DNA deaminase activity using Escherichia coli DNA as a reporter has been questioned, representing another round in the ongoing debate.

Original language	English (US)
Pages (from-to)	224-227
Number of pages	4
Journal	Trends in Genetics
Volume	20
Issue number	6
DOIs	https://doi.org/10.1016/j.tig.2004.03.012
State	Published - Jun 1 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/j.tig.2004.03.012

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title = "Activation induced deaminase: The importance of being specific",

abstract = "Activation-induced deaminase (AID) is required for class switch recombination and somatic hypermutation in immunoglobulin genes. Although the preponderance of evidence suggests that AID functions by deaminating deoxycytidine in DNA, the question remains whether it can also deaminate cytidine in mRNA, as originally proposed based on its homology to RNA-editing enzymes. Recently, the biological relevance of assaying mammalian enzymes for DNA deaminase activity using Escherichia coli DNA as a reporter has been questioned, representing another round in the ongoing debate.",

author = "Smith, {Harold C.} and Andrea Bottaro and Sowden, {Mark P.} and Wedekind, {Joseph E.}",

note = "Funding Information: HCS is supported by NIH grants DK43739, AI54369 and an AFOSR grant F49620. MPS is supported by NIH grants and an AFOSR grant F49620. AB is supported by NIH grant AI45012. JEW is supported by NIH grants RR15934 and GM63162 and by an HHMI pilot project grant 76200–537802.",

year = "2004",

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doi = "10.1016/j.tig.2004.03.012",

language = "English (US)",

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TY - JOUR

T1 - Activation induced deaminase

T2 - The importance of being specific

AU - Smith, Harold C.

AU - Bottaro, Andrea

AU - Sowden, Mark P.

AU - Wedekind, Joseph E.

N1 - Funding Information: HCS is supported by NIH grants DK43739, AI54369 and an AFOSR grant F49620. MPS is supported by NIH grants and an AFOSR grant F49620. AB is supported by NIH grant AI45012. JEW is supported by NIH grants RR15934 and GM63162 and by an HHMI pilot project grant 76200–537802.

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Activation-induced deaminase (AID) is required for class switch recombination and somatic hypermutation in immunoglobulin genes. Although the preponderance of evidence suggests that AID functions by deaminating deoxycytidine in DNA, the question remains whether it can also deaminate cytidine in mRNA, as originally proposed based on its homology to RNA-editing enzymes. Recently, the biological relevance of assaying mammalian enzymes for DNA deaminase activity using Escherichia coli DNA as a reporter has been questioned, representing another round in the ongoing debate.

AB - Activation-induced deaminase (AID) is required for class switch recombination and somatic hypermutation in immunoglobulin genes. Although the preponderance of evidence suggests that AID functions by deaminating deoxycytidine in DNA, the question remains whether it can also deaminate cytidine in mRNA, as originally proposed based on its homology to RNA-editing enzymes. Recently, the biological relevance of assaying mammalian enzymes for DNA deaminase activity using Escherichia coli DNA as a reporter has been questioned, representing another round in the ongoing debate.

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DO - 10.1016/j.tig.2004.03.012

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JO - Trends in Genetics

JF - Trends in Genetics

IS - 6

ER -

Activation induced deaminase: The importance of being specific

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