A useful spectrophotometric rate assay for pepsin

T. Peter Stein, Ted W. Reid, David Fahrney

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Phenyl sulfite is a specific substrate for pepsin, whose hydrolysis can be followed spectrophotometrically. This compound is superior to peptide substrates in its ease of synthesis and its ability to be monitered spectrophotometrically. The sulfite assay is therefore the method of choice for both routine and detailed assays of pepsin.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalAnalytical Biochemistry
Volume41
Issue number2
DOIs
StatePublished - Jun 1971

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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