A useful spectrophotometric rate assay for pepsin

T. Peter Stein, Ted W. Reid, David Fahrney

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Phenyl sulfite is a specific substrate for pepsin, whose hydrolysis can be followed spectrophotometrically. This compound is superior to peptide substrates in its ease of synthesis and its ability to be monitered spectrophotometrically. The sulfite assay is therefore the method of choice for both routine and detailed assays of pepsin.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalAnalytical Biochemistry
Issue number2
StatePublished - Jun 1971
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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