A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies

Quanzhi Li; M. E. DiSanto; Wayne E. Magee

doi:10.1016/0378-1135(92)90004-D

A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies

Quanzhi Li
, M. E. DiSanto
, Wayne E. Magee

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1×10⁸ heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.

Original language	English (US)
Pages (from-to)	29-41
Number of pages	13
Journal	Veterinary Microbiology
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0378-1135(92)90004-D
State	Published - Jul 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
General Veterinary

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10.1016/0378-1135(92)90004-D

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title = "A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies",

abstract = "A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1×108 heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.",

author = "Quanzhi Li and DiSanto, \{M. E.\} and Magee, \{Wayne E.\}",

year = "1992",

month = jul,

doi = "10.1016/0378-1135(92)90004-D",

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T1 - A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies

AU - Li, Quanzhi

AU - DiSanto, M. E.

AU - Magee, Wayne E.

PY - 1992/7

Y1 - 1992/7

N2 - A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1×108 heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.

AB - A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1×108 heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.

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JF - Veterinary Microbiology

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ER -

A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies

Abstract

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