Abstract
A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1×108 heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.
Original language | English (US) |
---|---|
Pages (from-to) | 29-41 |
Number of pages | 13 |
Journal | Veterinary Microbiology |
Volume | 32 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1992 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Microbiology
- General Veterinary