A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies

Quanzhi Li, M. E. DiSanto, Wayne E. Magee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1×108 heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.

Original languageEnglish (US)
Pages (from-to)29-41
Number of pages13
JournalVeterinary Microbiology
Volume32
Issue number1
DOIs
StatePublished - Jul 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • General Veterinary

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