A rapid electrochemical probe for DNA and electroactive ligand interaction

Cathy Yang; Sufen Wang; Kedar Mahajan; Patricia Jackson; John Schmalzel

A rapid electrochemical probe for DNA and electroactive ligand interaction

Cathy Yang, Sufen Wang, Kedar Mahajan, Patricia Jackson, John Schmalzel

Electrical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

A rapid method for investigating interactions between DNA and electroactive ligands based on an electrochemical equation for irreversible processes is designed. Binding constants (K) and sizes of binding sites (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in the experiments. It was found that the binding constant of Hoechst 33258, mitoxantrone and actinomycin D, were 2.1×10 ⁸, 8.9×10 ⁹ and 9.1×10 ⁹ cm ³ mol ^-1; and the sizes of their binding sites were 4, 3 and 8, respectively. This study provides a convenient and sensitive approach to estimating affinity parameters and outlining interactions between DNA and electroactive targeting compounds.

Original language	English (US)
Pages	8-13
Number of pages	6
State	Published - 2006
Event	2006 IEEE Sensors Applications Symposium - Houston, TX, United States Duration: Feb 7 2006 → Feb 9 2006

Other

Other	2006 IEEE Sensors Applications Symposium
Country/Territory	United States
City	Houston, TX
Period	2/7/06 → 2/9/06

All Science Journal Classification (ASJC) codes

General Engineering

Cite this

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title = "A rapid electrochemical probe for DNA and electroactive ligand interaction",

abstract = "A rapid method for investigating interactions between DNA and electroactive ligands based on an electrochemical equation for irreversible processes is designed. Binding constants (K) and sizes of binding sites (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in the experiments. It was found that the binding constant of Hoechst 33258, mitoxantrone and actinomycin D, were 2.1×10 8, 8.9×10 9 and 9.1×10 9 cm 3 mol -1; and the sizes of their binding sites were 4, 3 and 8, respectively. This study provides a convenient and sensitive approach to estimating affinity parameters and outlining interactions between DNA and electroactive targeting compounds.",

author = "Cathy Yang and Sufen Wang and Kedar Mahajan and Patricia Jackson and John Schmalzel",

year = "2006",

language = "English (US)",

pages = "8--13",

note = "2006 IEEE Sensors Applications Symposium ; Conference date: 07-02-2006 Through 09-02-2006",

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TY - CONF

T1 - A rapid electrochemical probe for DNA and electroactive ligand interaction

AU - Yang, Cathy

AU - Wang, Sufen

AU - Mahajan, Kedar

AU - Jackson, Patricia

AU - Schmalzel, John

PY - 2006

Y1 - 2006

N2 - A rapid method for investigating interactions between DNA and electroactive ligands based on an electrochemical equation for irreversible processes is designed. Binding constants (K) and sizes of binding sites (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in the experiments. It was found that the binding constant of Hoechst 33258, mitoxantrone and actinomycin D, were 2.1×10 8, 8.9×10 9 and 9.1×10 9 cm 3 mol -1; and the sizes of their binding sites were 4, 3 and 8, respectively. This study provides a convenient and sensitive approach to estimating affinity parameters and outlining interactions between DNA and electroactive targeting compounds.

AB - A rapid method for investigating interactions between DNA and electroactive ligands based on an electrochemical equation for irreversible processes is designed. Binding constants (K) and sizes of binding sites (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in the experiments. It was found that the binding constant of Hoechst 33258, mitoxantrone and actinomycin D, were 2.1×10 8, 8.9×10 9 and 9.1×10 9 cm 3 mol -1; and the sizes of their binding sites were 4, 3 and 8, respectively. This study provides a convenient and sensitive approach to estimating affinity parameters and outlining interactions between DNA and electroactive targeting compounds.

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M3 - Paper

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T2 - 2006 IEEE Sensors Applications Symposium

Y2 - 7 February 2006 through 9 February 2006

ER -

A rapid electrochemical probe for DNA and electroactive ligand interaction

Abstract

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All Science Journal Classification (ASJC) codes

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