A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer

G. S. Goldberg; J. F. Bechberger; C. C.G. Naus

A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer

G. S. Goldberg, J. F. Bechberger, C. C.G. Naus

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil. Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.

Original language	English (US)
Pages (from-to)	490-497
Number of pages	8
Journal	BioTechniques
Volume	18
Issue number	3
State	Published - 1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
General Biochemistry, Genetics and Molecular Biology

Cite this

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abstract = "We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil. Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.",

author = "Goldberg, {G. S.} and Bechberger, {J. F.} and Naus, {C. C.G.}",

year = "1995",

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AU - Bechberger, J. F.

AU - Naus, C. C.G.

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N2 - We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil. Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.

AB - We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil. Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.

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A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer

Abstract

All Science Journal Classification (ASJC) codes

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