A novel human xenograft model of inflammatory breast cancer

Mary L. Alpaugh, James S. Tomlinson, Zhi Ming Shao, Sanford H. Barsky

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The step of intravasation or lymphovascular invasion can be a rate- limiting step in the metastatic process. Inflammatory breast carcinoma manifests an exaggerated degree of lymphovascular invasion in situ; hence, a study of its molecular basis might shed light on the general mechanism of lymphovascular invasion exhibited by all metastasizing cancers. To this end, we have established the first human transplantable inflammatory breast carcinoma xenograft (MARY-X) in scid/nude mice. Whereas all other human xenografts grew as isolated s.c. nodules, MARY-X grew exclusively within murine lymphatics and blood vessels, and these latter elements and their supporting stroma comprised, by murine Cot-1 DNA analysis, 30% of the tumor. MARY-X, like its human counterpart, exhibited striking erythema of the overlying skin. MARY-X was estrogen receptor, progesterone receptor, Her- 2/neu negative and p53, epidermal growth factor receptor positive. The primary tumor of origin of MARY-X exhibited identical markers, except that about 50% of its cells exhibited Her-2/neu amplification. Comparative studies of MARY-X with noninflammatory xenografts indicated 10-20-fold overexpression of E-cadherin and MUC1, findings that were reflected in actual cases of human inflammatory breast cancer. MARY-X should allow us to further dissect out both the upstream regulatory machinery and the downstream effector molecules responsible for the inflammatory carcinoma phenotype.

Original languageEnglish (US)
Pages (from-to)5079-5084
Number of pages6
JournalCancer Research
Volume59
Issue number20
StatePublished - Oct 15 1999

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Inflammatory Breast Neoplasms
Heterografts
Neoplasms
Lymphatic Vessels
Progesterone Receptors
Cadherins
Erythema
Epidermal Growth Factor Receptor
Nude Mice
Estrogen Receptors
Blood Vessels
Carcinoma
Phenotype
Skin
DNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Alpaugh, M. L., Tomlinson, J. S., Shao, Z. M., & Barsky, S. H. (1999). A novel human xenograft model of inflammatory breast cancer. Cancer Research, 59(20), 5079-5084.
Alpaugh, Mary L. ; Tomlinson, James S. ; Shao, Zhi Ming ; Barsky, Sanford H. / A novel human xenograft model of inflammatory breast cancer. In: Cancer Research. 1999 ; Vol. 59, No. 20. pp. 5079-5084.
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Alpaugh, ML, Tomlinson, JS, Shao, ZM & Barsky, SH 1999, 'A novel human xenograft model of inflammatory breast cancer', Cancer Research, vol. 59, no. 20, pp. 5079-5084.

A novel human xenograft model of inflammatory breast cancer. / Alpaugh, Mary L.; Tomlinson, James S.; Shao, Zhi Ming; Barsky, Sanford H.

In: Cancer Research, Vol. 59, No. 20, 15.10.1999, p. 5079-5084.

Research output: Contribution to journalArticle

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Alpaugh ML, Tomlinson JS, Shao ZM, Barsky SH. A novel human xenograft model of inflammatory breast cancer. Cancer Research. 1999 Oct 15;59(20):5079-5084.