A Modified Enzymatic Method For The Estimation of Dihydroxyacetone

A. K. Ghosh; N. S. Vasan; Sabita Ghosh

doi:10.1080/00032717508059071

A Modified Enzymatic Method For The Estimation of Dihydroxyacetone

A. K. Ghosh
, N. S. Vasan
, Sabita Ghosh

Research output: Contribution to journal › Article › peer-review

Abstract

A two stage assay procedure has been developed for the estimation of dihydroxyacetone (DIIA). DIIA is at first phosphorylated to dihydroxyacetone phosphate (DHAP) by glycerokinase (GK) and ATP at pH 8.5. The DHAP formed is then measured enzymatically using glycerol-3P dehydrogenase (GDI!) and NADH at pH 7.6. The sensitivity of this method is found to be adequate for both routine assays and kinetic studies.

Original language	English (US)
Pages (from-to)	939-947
Number of pages	9
Journal	Analytical Letters
Volume	8
Issue number	12
DOIs	https://doi.org/10.1080/00032717508059071
State	Published - Dec 1 1975
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032717508059071

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abstract = "A two stage assay procedure has been developed for the estimation of dihydroxyacetone (DIIA). DIIA is at first phosphorylated to dihydroxyacetone phosphate (DHAP) by glycerokinase (GK) and ATP at pH 8.5. The DHAP formed is then measured enzymatically using glycerol-3P dehydrogenase (GDI!) and NADH at pH 7.6. The sensitivity of this method is found to be adequate for both routine assays and kinetic studies.",

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N2 - A two stage assay procedure has been developed for the estimation of dihydroxyacetone (DIIA). DIIA is at first phosphorylated to dihydroxyacetone phosphate (DHAP) by glycerokinase (GK) and ATP at pH 8.5. The DHAP formed is then measured enzymatically using glycerol-3P dehydrogenase (GDI!) and NADH at pH 7.6. The sensitivity of this method is found to be adequate for both routine assays and kinetic studies.

AB - A two stage assay procedure has been developed for the estimation of dihydroxyacetone (DIIA). DIIA is at first phosphorylated to dihydroxyacetone phosphate (DHAP) by glycerokinase (GK) and ATP at pH 8.5. The DHAP formed is then measured enzymatically using glycerol-3P dehydrogenase (GDI!) and NADH at pH 7.6. The sensitivity of this method is found to be adequate for both routine assays and kinetic studies.

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JO - Analytical Letters

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A Modified Enzymatic Method For The Estimation of Dihydroxyacetone

Abstract

All Science Journal Classification (ASJC) codes

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