TY - JOUR
T1 - A method for studying the three‐dimensional organization of cytoskeletal elements of cells
T2 - improvements in the polyethylene glycol technique
AU - Nagele, R. G.
AU - Roisen, F. J.
AU - Lee, H.
PY - 1983/2
Y1 - 1983/2
N2 - A method utilizing polyethylene glycol (PEG) as an extractable embedment for electron microscopy is described. Tissues are fixed according to conventional protocols, embedded in PEG, and sectioned. Sections (ranging from 100 to 500 nm in thickness) are mounted on grids, divested of their PEG matrix, critical‐point‐dried, and examined stereoscopically. This method greatly facilitates studies on the three‐dimensional organization of cytoskeletal and cytoplasmic contractile systems in both muscle and nonmuscle cells. 1983 Blackwell Science Ltd
AB - A method utilizing polyethylene glycol (PEG) as an extractable embedment for electron microscopy is described. Tissues are fixed according to conventional protocols, embedded in PEG, and sectioned. Sections (ranging from 100 to 500 nm in thickness) are mounted on grids, divested of their PEG matrix, critical‐point‐dried, and examined stereoscopically. This method greatly facilitates studies on the three‐dimensional organization of cytoskeletal and cytoplasmic contractile systems in both muscle and nonmuscle cells. 1983 Blackwell Science Ltd
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U2 - 10.1111/j.1365-2818.1983.tb04172.x
DO - 10.1111/j.1365-2818.1983.tb04172.x
M3 - Article
C2 - 6682450
AN - SCOPUS:0020678678
SN - 0022-2720
VL - 129
SP - 179
EP - 184
JO - Journal of Microscopy
JF - Journal of Microscopy
IS - 2
ER -