A method for studying the three‐dimensional organization of cytoskeletal elements of cells: improvements in the polyethylene glycol technique

R. G. Nagele, F. J. Roisen, H. Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A method utilizing polyethylene glycol (PEG) as an extractable embedment for electron microscopy is described. Tissues are fixed according to conventional protocols, embedded in PEG, and sectioned. Sections (ranging from 100 to 500 nm in thickness) are mounted on grids, divested of their PEG matrix, critical‐point‐dried, and examined stereoscopically. This method greatly facilitates studies on the three‐dimensional organization of cytoskeletal and cytoplasmic contractile systems in both muscle and nonmuscle cells. 1983 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)179-184
Number of pages6
JournalJournal of Microscopy
Volume129
Issue number2
DOIs
StatePublished - Feb 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

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