A method for exposing the internal anatomy of small and delicate tissues for correlated SEM/TEM studies using polyethylene glycol embedding

R. G. Nagele; K. J. Doane; H. Lee; F. J. Wilson; F. J. Roisen

doi:10.1111/j.1365-2818.1984.tb00483.x

A method for exposing the internal anatomy of small and delicate tissues for correlated SEM/TEM studies using polyethylene glycol embedding

R. G. Nagele, K. J. Doane, H. Lee, F. J. Wilson, F. J. Roisen

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Abstract

A method for preparing and handling large, clean, distortion‐free cut surfaces through small and delicate tissues for correlated SEM/TEM examination is described. In this method, tissues are fixed according to conventional protocols; however, instead of critical‐point‐drying after fixation, tissues are first embedded in polyethylene glycol (PEG), a water‐soluble waxy solid. Tissue blocks are easily oriented and sectioned to the desired regions, immersed in a solvent to remove PEG, critical‐point‐dried, and examined with an SEM. The same tissue blocks can be reworked for TEM by immersing in propylene oxide and embedding in an epoxy resin. 1984 Blackwell Science Ltd

Original language	English (US)
Pages (from-to)	177-183
Number of pages	7
Journal	Journal of Microscopy
Volume	133
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2818.1984.tb00483.x
State	Published - Feb 1984
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology

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10.1111/j.1365-2818.1984.tb00483.x

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title = "A method for exposing the internal anatomy of small and delicate tissues for correlated SEM/TEM studies using polyethylene glycol embedding",

abstract = "A method for preparing and handling large, clean, distortion‐free cut surfaces through small and delicate tissues for correlated SEM/TEM examination is described. In this method, tissues are fixed according to conventional protocols; however, instead of critical‐point‐drying after fixation, tissues are first embedded in polyethylene glycol (PEG), a water‐soluble waxy solid. Tissue blocks are easily oriented and sectioned to the desired regions, immersed in a solvent to remove PEG, critical‐point‐dried, and examined with an SEM. The same tissue blocks can be reworked for TEM by immersing in propylene oxide and embedding in an epoxy resin. 1984 Blackwell Science Ltd",

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year = "1984",

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AU - Nagele, R. G.

AU - Doane, K. J.

AU - Lee, H.

AU - Wilson, F. J.

AU - Roisen, F. J.

PY - 1984/2

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N2 - A method for preparing and handling large, clean, distortion‐free cut surfaces through small and delicate tissues for correlated SEM/TEM examination is described. In this method, tissues are fixed according to conventional protocols; however, instead of critical‐point‐drying after fixation, tissues are first embedded in polyethylene glycol (PEG), a water‐soluble waxy solid. Tissue blocks are easily oriented and sectioned to the desired regions, immersed in a solvent to remove PEG, critical‐point‐dried, and examined with an SEM. The same tissue blocks can be reworked for TEM by immersing in propylene oxide and embedding in an epoxy resin. 1984 Blackwell Science Ltd

AB - A method for preparing and handling large, clean, distortion‐free cut surfaces through small and delicate tissues for correlated SEM/TEM examination is described. In this method, tissues are fixed according to conventional protocols; however, instead of critical‐point‐drying after fixation, tissues are first embedded in polyethylene glycol (PEG), a water‐soluble waxy solid. Tissue blocks are easily oriented and sectioned to the desired regions, immersed in a solvent to remove PEG, critical‐point‐dried, and examined with an SEM. The same tissue blocks can be reworked for TEM by immersing in propylene oxide and embedding in an epoxy resin. 1984 Blackwell Science Ltd

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A method for exposing the internal anatomy of small and delicate tissues for correlated SEM/TEM studies using polyethylene glycol embedding

Abstract

All Science Journal Classification (ASJC) codes

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