A method for exposing the internal anatomy of small and delicate tissues for correlated SEM/TEM studies using polyethylene glycol embedding

R. G. Nagele, K. J. Doane, H. Lee, F. J. Wilson, F. J. Roisen

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A method for preparing and handling large, clean, distortion‐free cut surfaces through small and delicate tissues for correlated SEM/TEM examination is described. In this method, tissues are fixed according to conventional protocols; however, instead of critical‐point‐drying after fixation, tissues are first embedded in polyethylene glycol (PEG), a water‐soluble waxy solid. Tissue blocks are easily oriented and sectioned to the desired regions, immersed in a solvent to remove PEG, critical‐point‐dried, and examined with an SEM. The same tissue blocks can be reworked for TEM by immersing in propylene oxide and embedding in an epoxy resin. 1984 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalJournal of Microscopy
Volume133
Issue number2
DOIs
StatePublished - Feb 1984

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

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