TY - JOUR
T1 - A highly adhesive and naturally derived sealant
AU - Assmann, Alexander
AU - Vegh, Andrea
AU - Ghasemi-Rad, Mohammad
AU - Bagherifard, Sara
AU - Cheng, George
AU - Sani, Ehsan Shirzaei
AU - Ruiz-Esparza, Guillermo U.
AU - Noshadi, Iman
AU - Lassaletta, Antonio D.
AU - Gangadharan, Sidhu
AU - Tamayol, Ali
AU - Khademhosseini, Ali
AU - Annabi, Nasim
N1 - Funding Information:
The authors gratefully acknowledge Lay-Hong Ang for her work and support regarding histological and immunohistological readout, and Mina Keshvardoost for her work in the degradation experiments. AA acknowledges postdoctoral funding from the German Heart Foundation, Frankfurt, Germany. SB acknowledges funding from MIT-Italy program (Progetto Rocca) and Polimi International Fellowship (PIF). The authors acknowledge funding from the National Institutes of Health (AR057837, DE021468, D005865, AR068258, AR066193, EB022403, EB021148), and the Office of Naval Research Presidential Early Career Award for Scientists and Engineers (PECASE). N.A. acknowledges the support from the American Heart Association (AHA, 16SDG31280010), FY17 TIER 1 Interdisciplinary Research Seed Grants from Northeastern University, and the startup fund provided by the Department of Chemical Engineering, College of Engineering at Northeastern University.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/9
Y1 - 2017/9
N2 - Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.
AB - Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.
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U2 - 10.1016/j.biomaterials.2017.06.004
DO - 10.1016/j.biomaterials.2017.06.004
M3 - Article
C2 - 28646685
AN - SCOPUS:85021071764
VL - 140
SP - 115
EP - 127
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
ER -