TY - JOUR
T1 - A gel aspiration-ejection system for the controlled production and delivery of injectable dense collagen scaffolds
AU - Kamranpour, Neysan O.
AU - Miri, Amir K.
AU - James-Bhasin, Mark
AU - Nazhat, Showan N.
N1 - Funding Information:
Funding of CIHR, NSERC, CFI, Quebec MESRST (MDEIE), and McGill University Faculty of Engineering Gerald Hatch Faculty Fellowship are gratefully acknowledged. The authors acknowledge Imaging and Molecular Biology Platform center (Pharmacology and Therapeutics Department, McGill University, Montreal) and thank Dr Wolfgang Reintsch for his technical assistance in NLSM imaging. The authors also acknowledge Dr Naser Muja for his technical assistance in recording the supplemental video.
Publisher Copyright:
© 2016 IOP Publishing Ltd.
PY - 2016
Y1 - 2016
N2 - A gel aspiration-ejection (GAE) system has been developed for the advanced production and delivery of injectable dense collagen (I-DC) gels of unique collagen fibrillar densities (CFDs). Through the creation of negative pressure, GAE aspirates prefabricated highly hydrated collagen gels into a needle, simultaneously inducing compaction and meso-scale anisotropy (i.e., fibrillar alignment) on the gels, and by subsequent reversal of the pressure, I-DC gels can be controllably ejected. The system generates I-DC gels with CFDs ranging from 5 to 32 wt%, controlling the initial scaffold microstructure, anisotropy, hydraulic permeability, and mechanical properties. These features could potentially enable the minimally invasive delivery of more stable hydrogels. The viability, metabolic activity, and differentiation of seeded mesenchymal stem cells (MSCs) was investigated in the I-DC gels of distinct CFDs and extents of anisotropy produced through two different gauge needles. MSC osteoblastic differentiation was found to be relatively accelerated in I-DC gels that combined physiologically relevant CFDs and increased fibrillar alignment. The ability to not only support homogenous cell seeding, but also to direct and accelerate their differentiation through tissue-equivalent anisotropy, creates numerous opportunities in regenerative medicine.
AB - A gel aspiration-ejection (GAE) system has been developed for the advanced production and delivery of injectable dense collagen (I-DC) gels of unique collagen fibrillar densities (CFDs). Through the creation of negative pressure, GAE aspirates prefabricated highly hydrated collagen gels into a needle, simultaneously inducing compaction and meso-scale anisotropy (i.e., fibrillar alignment) on the gels, and by subsequent reversal of the pressure, I-DC gels can be controllably ejected. The system generates I-DC gels with CFDs ranging from 5 to 32 wt%, controlling the initial scaffold microstructure, anisotropy, hydraulic permeability, and mechanical properties. These features could potentially enable the minimally invasive delivery of more stable hydrogels. The viability, metabolic activity, and differentiation of seeded mesenchymal stem cells (MSCs) was investigated in the I-DC gels of distinct CFDs and extents of anisotropy produced through two different gauge needles. MSC osteoblastic differentiation was found to be relatively accelerated in I-DC gels that combined physiologically relevant CFDs and increased fibrillar alignment. The ability to not only support homogenous cell seeding, but also to direct and accelerate their differentiation through tissue-equivalent anisotropy, creates numerous opportunities in regenerative medicine.
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U2 - 10.1088/1758-5090/8/1/015018
DO - 10.1088/1758-5090/8/1/015018
M3 - Article
C2 - 27003606
AN - SCOPUS:85003048894
VL - 8
JO - Biofabrication
JF - Biofabrication
SN - 1758-5082
IS - 1
M1 - 015018
ER -