A connexin 43 antisense vector reduces the ability of normal cells to inhibit the foci formation of transformed cells

Gary S. Goldberg, Kendra Dean Martyn, Alan F. Lau

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Antisense gene constructs have been very useful in the functional analysis of genes and their products. In this report we used a connexin 43 (Cx43) antisense gene construct to study the role that heterologous gap‐junctional intracellular communication (GJIC) plays in the ability of untransformed fibroblasts to suppress the foci‐forming ability of src oncogene‐transformed cells. Untransformed Rat‐1 fibroblasts transfected with the Cx43 antisenese DNA construct showed marked decreases in Cx43 RNA and protein, which were accompanied by a corresponding decrease in GJIC. These Cx43 antisense‐transfected cells maintained normal cell morphology, growth rates, and saturation densities and did not grow in soft‐agar suspension. However, in coculture experiments, the Cx43 antisense cells were less effective than vector‐alone‐transfected, sense‐trans‐fected, and untransfected cells at in hibiting foci formation of pp60v‐src‐transformed cells. These effects of junctionally competent, normal cells were associated with the existence of heterologous GJIC with the transformed cells and did not appear to result from the elaboration of a stable, diffusible inhibitory factor. Thus, gap‐junction‐mediated transfer of putative regulatory molecules may play a role in the ability of untransformed cells to suppress the expression of certain properties of transformed cells. ©1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)106-114
Number of pages9
JournalMolecular Carcinogenesis
Volume11
Issue number2
DOIs
StatePublished - Oct 1994

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cancer Research

Fingerprint Dive into the research topics of 'A connexin 43 antisense vector reduces the ability of normal cells to inhibit the foci formation of transformed cells'. Together they form a unique fingerprint.

Cite this